Protein_Domain

Part:BBa_K3264003:Design

Designed by: Xinyou Chang   Group: iGEM19_GreatBay_SZ   (2019-10-16)


Rep


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We coded the nucleotide sequence of 2Rep, a repetitive part consisting of two polyalanine and glycine-rich repeat regions from MaSp1 of E. australis (GNSGRGQGGYGQGSGGNAAAAAAAAAAAAAAAGQGGQGGYGR QSQGAGSAAAAAAAAAAAAAAGSGQGGYGGQGQGGYGQSGNS) Also, the linker between the NT/CT and the 2Rep(repetitive region) is GNS The construct was cloned into a pT7 plasmid and transformed into BL21 (DE3) E. coli together with NT-2Rep-CT or NT-4Rep-CT


Source

de novo synthesize DNA from Euprosthenops australis

References